Hypoxia Methods and Protocols 1st edition 2018
Oxygen is an essential molecule that serves as an enzyme substrate in bioenergetics and metabolic reactions. Adaptation to oxygen deprivation—hypoxia—is a critical process in development, physiology, and pathophysiology. This Hypoxia Methods and Protocols 1st edition 2018 offers a comprehensive coverage of step-by-step lab protocols that are widely used and those that are developed more recently for studying hypoxic responses in physiology and diseases.
All the protocols contain extensive detail intended to help competent scientists in the field of biomedical sciences carry out unfamiliar molecular biology techniques successfully by simply following the detailed procedures. Practical notes are explained in greater detail for additional help.
Methods included range from the application of hypoxic conditions to the techniques of investigating hydroxylase activities with various approaches including CRISPR/Cas9, substrate hydroxylation with mass spectrometry, transcriptional activation of hypoxia-response genes with a dynamic luciferase assay, chromatin immunoprecipitation, and RNA sequencing, metabolic changes in vitro and in vivo, sensing of hypoxia signal in animal, placental and erythrocyte development, hypoxic responses in C. elegans and D. rerio, and in tumor tissues, manipulation of hypoxic responses with RCAS/TVA tumor models, cancer-stem cell enrichment and quantification, and tumor growth with various imaging modalities. This Hypoxia Methods and Protocols 1st edition 2018 provides a valuable set of tools that can be utilized to study hypoxia and beyond.